De Beer is a Dutch and Afrikaans surname, meaning "the bear". It can refer to:
Esmond Samuel de Beer (1895–1990), New Zealand bibliophile, art collector and philanthropist
Gavin de Beer (1899–1972), British evolutionary embryologist
de:Hans de Beer (born 1957), Dutch illustrator and author
Jan de Beer (c.1475–1528), Flemish painter and draughtsman
Jannie de Beer (born 1971), South African rugby player
Joos de Beer (<1535-1591), Dutch painter
Lonell de Beer (born 1980), South African cricketer
Racheltjie de Beer (1831-1843), Afrikaner heroine who may be fictional
Sue de Beer (born 1973), American contemporary artist
Willem de Beer (born 1988), South African sprinter
Wolfgang de Beer (born 1964), German footballer
Zach de Beer (1928–1999), South African politician and businessman
