Entrez 81620 | Ensembl ENSG00000167513 | |
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Aliases CDT1, DUP, RIS2, chromatin licensing and DNA replication factor 1 External IDs MGI: 1914427 HomoloGene: 32650 GeneCards: CDT1 | ||
DNA replication factor Cdt1 is a protein that in humans is encoded by the CDT1 gene.
Contents
The protein encoded by this gene is a key licensing factor which, along with the protein Cdc6, functions to license DNA by forming the pre-replication complex (pre-RC). Its activity during the cell cycle is tightly regulated by its association with the protein geminin, which both inhibits Cdt1 activity during S phase in order to prevent re-replication of DNA and prevents it from ubiquitination and subsequent proteolysis.
Orthologs
CDT1 belongs to a family of replication proteins conserved from yeast to humans. Examples of orthologs in other species include:
Interactions
DNA replication factor CDT1 has been shown to interact with SKP2. Cdt1 is recruited by the origin recognition complex in origin licensing. Null-mutations for Cdt1 are lethal in yeast; the spores undergo mitosis without DNA replication. The overexpression of Cdt1 causes rereplication in H. sapiens, which activates the Chk1 pathway, preventing entry into mitosis.