D benzoylarginine 4 nitroanilide amidase

In enzymology, a D-benzoylarginine-4-nitroanilide amidase (EC 3.5.1.72) is an enzyme that catalyzes the chemical reaction

N-benzoyl-D-arginine-4-nitroanilide + H₂O ⇌ N-benzoyl-D-arginine + 4-nitroaniline

Thus, the two substrates of this enzyme are N-benzoyl-D-arginine-4-nitroanilide and H₂O, whereas its two products are N-benzoyl-D-arginine and 4-nitroaniline.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-benzoyl-D-arginine-4-nitroanilide amidohydrolase. Other names in common use include benzoyl-D-arginine arylamidase, and D-BAPA-ase.