Cryo bio-crystallography is the application of crystallography at cryogenic temperatures.
Cryo bio-crystallography Wikipedia
Cryo crystallography enables X-ray data collection at cryogenic, near liquid nitrogen temperatures (also called: N2).
- Crystals are transferred from the solution they have grown in (called mother liquor) to a hydrocarbon environment
- Crystals are mounted with a glass fiber (as opposed to a capillary)
- Crystals are cooled with a cold nitrogen stream on a diffraction apparatus to prevent the solvent freezing in the crystals thus maintaining crystallographic integrity.
- Significant improvement of resolution in data collection
- Reduced or eliminated radiation damage in crystals
Crystallography of large biological macromolecules can be achieved while maintaining their solution state. The most known example is the ribosome.