EC number 3.5.2.10 IntEnz IntEnz view ExPASy NiceZyme view | CAS number 9025-13-2 BRENDA BRENDA entry KEGG KEGG entry | |
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In enzymology, a creatininase (EC 3.5.2.10) is an enzyme that catalyses the hydrolysis of creatinine to creatine, which can then be metabolised to urea and sarcosine by creatinase.
creatinine + H2OThus, the two substrates of this enzyme are creatinine and H2O, whereas its product is creatine.
Creatininase is a member of the urease-related amidohydrolases, the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is creatinine amidohydrolase. This enzyme is also called creatinine hydrolase. This enzyme participates in arginine and proline metabolism.
Structural studies
Creatininase from Pseudomonas putida has a core structure consisting of 3-layers, alpha/beta/alpha.
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1J2T, 1J2U, 1Q3K, and 1V7Z.