Choloyl CoA hydrolase

In enzymology, a choloyl-CoA hydrolase (EC 3.1.2.27) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are choloyl-CoA and H₂O, whereas its two products are cholate and CoA.

This enzyme belongs to the family of hydrolases, specifically those acting on thioester bonds. The systematic name of this enzyme class is choloyl-CoA hydrolase. Other names in common use include PTE-2 (ambiguous), choloyl-coenzyme A thioesterase, chenodeoxycholoyl-coenzyme A thioesterase, and peroxisomal acyl-CoA thioesterase 2.