Species Human Entrez 771 | Human Mouse Ensembl ENSG00000074410 | |
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Aliases CA12, CAXII, HsT18816, CA-XII, T18816, carbonic anhydrase 12 External IDs MGI: 1923709 HomoloGene: 20327 GeneCards: CA12 | ||
Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene.
Contents
Function
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.
Pathology
Loss of function mutations in the CAXII gene result in defects in fluids and carbonate secretions in the following diseases:
1) Cystic fibrosis-like syndrome with normal cystic fibrosis transmembrane conductance regulator (CFTR) protein levels
2) Pancreatitis
3) Sjögren's syndrome
4) Xerostomia or dry mouth syndrome
Molecular Basis of Cystic Fibrosis-like Syndrome
CAXII, with either the His121Gln or Glu143Lys mutation, localizes to basolateral membranes of polarized MDCK cells similar to the wild type enzyme, indicating no deleterious effect on subcellular location.
However, CAXII mutant enzymes show reduced activity. These observations made it very hard to explain the mechanism for the autosomal recessive disorder of hyponatremia, causing salt wasting in sweat due to mutant CAXII.
In a separate study, researchers observed that mutant enzyme activity is completely reduced at physiological concentrations of sodium chloride. Thus, loss of the function of CAXII in sweat glands and lungs is the molecular basis for cystic fibrosis patients with normal CFTR levels.
High Impact Information on CAXII
Differential modulation of the active site environment of CAXII by cationic quantum dots and polylysine helps design CAXII specific activators and inhibitors of the enzyme. CAXII specific inhibition provides a tool to interfere with cell proliferation, resulting in cell apoptosis in T-cell lymphomas.
Analytical, Diagnostic, and Therapeutic Context of CAXII
Serum CAXII levels should be applicable as a sero-diagnostic marker for lung cancer.