Arylacetonitrilase

In enzymology, an arylacetonitrilase (EC 3.5.5.5) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are 4-chlorophenylacetonitrile and H₂O, whereas its two products are 4-chlorophenylacetate and NH₃.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in nitriles. The systematic name of this enzyme class is arylacetonitrile aminohydrolase. This enzyme participates in cyanoamino acid metabolism.