Allophanate hydrolase

In enzymology, an allophanate hydrolase (EC 3.5.1.54) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are allophanate (urea-1-carboxylate or N-carbamoylcarbamate) and H₂O, whereas its two products are HCO₃⁻ and NH₄⁺.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is urea-1-carboxylate amidohydrolase. This enzyme is also called allophanate lyase. This enzyme participates in urea cycle and metabolism of amino groups and atrazine degradation.