Acetoacetate—CoA ligase

In enzymology, an acetoacetate-CoA ligase (EC 6.2.1.16) is an enzyme that catalyzes the chemical reaction

The 3 substrates of this enzyme are ATP, acetoacetate, and CoA, whereas its 3 products are AMP, diphosphate, and acetoacetyl-CoA.

This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. The systematic name of this enzyme class is acetoacetate:CoA ligase (AMP-forming). This enzyme is also called acetoacetyl-CoA synthetase. This enzyme participates in butanoate metabolism.