The 2016 Internazionali BNL d'Italia (also known as the 2016 Italian Open and the 2016 Rome Masters) was a tennis tournament played on outdoor clay courts at the Foro Italico in Rome, Italy. It was the 73rd edition of the Italian Open and is classified as an ATP World Tour Masters 1000 event on the 2016 ATP World Tour and a Premier 5 event on the 2016 WTA Tour. It took place from 9–15 May 2016.
Rankings are as of May 2, 2016.
The following players received wildcards into the main draw:
 Salvatore Caruso
 Marco Cecchinato
 Paolo Lorenzi
 Lorenzo Sonego
The following players received entry from the qualifying draw:
 Aljaž Bedene
 Íñigo Cervantes
 Damir Džumhur
 Ernests Gulbis
 Mikhail Kukushkin
 Stéphane Robert
 Filippo Volandri
The following player received entry as a lucky loser:
 Lucas Pouille
Before the tournament
 Marcos Baghdatis →replaced by  Nicolas Mahut
 Marin Čilić →replaced by  Andreas Seppi
 Juan Martín del Potro →replaced by  Vasek Pospisil
 Tommy Haas →replaced by  Borna Ćorić
 John Isner →replaced by  Juan Mónaco
 Martin Kližan →replaced by  Teymuraz Gabashvili
 Gilles Simon →replaced by  Albert Ramos-Viñolas
 Jo-Wilfried Tsonga (muscle strain) →replaced by  Alexander Zverev
During the tournament
 Juan Mónaco
 Bernard Tomic (influenza)
Rankings are as of May 2, 2016.
The following pairs received wildcards into the doubles main draw:
 Andrea Arnaboldi /  Alessandro Giannessi
 Fabio Fognini /  Andreas Seppi
The following pair received entry as alternates:
 Dominic Inglot /  Fabrice Martin
Before the tournament
 Pierre-Hugues Herbert (knee injury)
 Kevin Anderson (muscle strain)
Rankings are as of May 2, 2016.
The following players received wildcards into the main draw:
 Claudia Giovine
 Karin Knapp
 Francesca Schiavone
The following players received entry from the qualifying draw:
 Kiki Bertens
 Mariana Duque Mariño
 Julia Görges
 Johanna Larsson
 Christina McHale
 Mónica Puig
 Alison Riske
 Heather Watson
Before the tournament
 Belinda Bencic (lower back injury) →replaced by Teliana Pereira
 Camila Giorgi (back injury) →replaced by Caroline Garcia
 Sloane Stephens →replaced by Tímea Babos
 Caroline Wozniacki (right ankle injury) →replaced by Yanina Wickmayer
Rankings are as of May 2, 2016.
The following pairs received wildcards into the doubles main draw:
 Claudia Giovine /  Angelica Moratelli
 Karin Knapp /  Francesca Schiavone
 Svetlana Kuznetsova /  Anastasia Pavlyuchenkova
 Serena Williams /  Venus Williams
 Andy Murray def.  Novak Djokovic, 6–3, 6–3
 Serena Williams def.  Madison Keys, 7–6(7–5), 6–3
 Bob Bryan /  Mike Bryan def.  Vasek Pospisil /  Jack Sock, 2–6, 6–3, [10–7]
 Martina Hingis /  Sania Mirza def.  Ekaterina Makarova /  Elena Vesnina, 6–1, 6–7(5–7), [10–3]