2000–01 Isthmian League

Premier Division

The Premier Division consisted of 22 clubs, including 18 clubs from the previous season and four new clubs:

Farnborough Town won the division and returned to the Football Conference after two seasons spent in Isthmian League. Slough Town, Carshalton Athletic and Dulwich Hamlet finished bottom of the table and relegated to the First Division. Three fixtures left unfulfilled due to bad weather conditions.

League table

Source:
^† Unfulfilled fixtures: Aldershot Town v Billericay Town, Hendon v Harrow Borough and Sutton United v Hendon.
Rules for classification: 1) points; 2) goal difference; 3) number of goals scored
(C) = Champion; (R) = Relegated; (P) = Promoted; (E) = Eliminated; (O) = Play-off winner; (A) = Advances to a further round.
Only applicable when the season is not finished:
(Q) = Qualified to the phase of tournament indicated; (TQ) = Qualified to tournament, but not yet to the particular phase indicated; (RQ) = Qualified to the relegation tournament indicated; (DQ) = Disqualified from tournament.

First Division

The First Division consisted of 22 clubs, including 17 clubs from the previous season and five new clubs: Three clubs relegated from the Premier Division:

Two clubs promoted from the Second Division:

Initially, champions of the Second Division Hemel Hempstead Town were to be promoted to the First Division, but later they were refused due to ground grading. Leatherhead finished in the relegation zone were reprieved.

Boreham Wood won the division and returned to the Premier Division at the first attempt. Bedford Town and Braintree Town also get a promotion. Leatherhead finished in the relegation zone the second time in a row and were relegated along with Romford and Barton Rovers.

League table

Source:
Rules for classification: 1) points; 2) goal difference; 3) number of goals scored
(C) = Champion; (R) = Relegated; (P) = Promoted; (E) = Eliminated; (O) = Play-off winner; (A) = Advances to a further round.
Only applicable when the season is not finished:
(Q) = Qualified to the phase of tournament indicated; (TQ) = Qualified to tournament, but not yet to the particular phase indicated; (RQ) = Qualified to the relegation tournament indicated; (DQ) = Disqualified from tournament.

Second Division

Second Division consisted of 22 clubs, including 17 clubs from the previous season and five new teams:

Two clubs relegated from the First Division:

Three clubs promoted from the Third Division:

Initially, champions of the Second Division Hemel Hempstead Town were to be promoted to the First Division, but later they were refused due to ground grading and stayed in the division.

Tooting & Mitcham United won the division and were promoted to the First Division along with Windsor & Eton and Barking. Later, Barking ceased to exist when they merged with East Ham United to form Barking & East Ham United, taken place of Barking in the First Division.

League table

Source:
^¶ At the end of the season Barking ceased to exist when they merged with East Ham United to form Barking & East Ham United.
^# Dispute between sources: says East Thurrock GF=72; Leighton Town GA=87, but says East Thurrock GF=71; Leighton Town GA=86.
^† Wokingham Town deducted one point.
Rules for classification: 1) points; 2) goal difference; 3) number of goals scored
(C) = Champion; (R) = Relegated; (P) = Promoted; (E) = Eliminated; (O) = Play-off winner; (A) = Advances to a further round.
Only applicable when the season is not finished:
(Q) = Qualified to the phase of tournament indicated; (TQ) = Qualified to tournament, but not yet to the particular phase indicated; (RQ) = Qualified to the relegation tournament indicated; (DQ) = Disqualified from tournament.

Third Division

Third Division consisted of 22 clubs, including 17 clubs from the previous season and five new teams:

Arlesey Town and Ashford Town both debutated in the league and achieved a promotion along with Lewes. Two fixtures left unfulfilled due to bad weather conditions.

League table

Source:
^¶ Unfulfilled fixtures: Croydon Athletic v Bracknell Town and Lewes v Hertford Town.
^† Abingdon Town deducted three points.
Rules for classification: 1) points; 2) goal difference; 3) number of goals scored
(C) = Champion; (R) = Relegated; (P) = Promoted; (E) = Eliminated; (O) = Play-off winner; (A) = Advances to a further round.
Only applicable when the season is not finished:
(Q) = Qualified to the phase of tournament indicated; (TQ) = Qualified to tournament, but not yet to the particular phase indicated; (RQ) = Qualified to the relegation tournament indicated; (DQ) = Disqualified from tournament.