2 (hydroxymethyl) 3 (acetamidomethylene)succinate hydrolase

In enzymology, a 2-(hydroxymethyl)-3-(acetamidomethylene)succinate hydrolase (EC 3.5.1.66) is an enzyme that catalyzes the chemical reaction

2-(hydroxymethyl)-3-(acetamidomethylene)succinate + 2 H₂O ⇌ acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH₃ + CO₂

Thus, the two substrates of this enzyme are 2-(hydroxymethyl)-3-(acetamidomethylene)succinate and H₂O, whereas its 4 products are acetate, 2-(hydroxymethyl)-4-oxobutanoate, NH₃, and CO₂.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is 2-(hydroxymethyl)-3-(acetamidomethylene)succinate amidohydrolase (deaminating, decarboxylating). Other names in common use include compound B hydrolase, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid, and hydrolase. This enzyme participates in vitamin B₆ metabolism.