2 (acetamidomethylene)succinate hydrolase

In enzymology, a 2-(acetamidomethylene)succinate hydrolase (EC 3.5.1.29) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are 2-(acetamidomethylene)succinate and H₂O, whereas its 4 products are acetate, succinate semialdehyde, NH₃, and CO₂.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is 2-(acetamidomethylene)succinate amidohydrolase (deaminating, decarboxylating). This enzyme is also called alpha-(N-acetylaminomethylene)succinic acid hydrolase. This enzyme participates in vitamin B₆ metabolism.