In the 1980–81 Beta Ethniki season was 22nd edition of second tier nationwide championship of Greece. Forty teams participated in the league, divided in two groups, the South and the North Group. Iraklis was named champion of the North Group and got promoted to the Alpha Ethniki. In the South Group Egaleo and Rodos tied and a play-off match, that found Rodos victorious by a 1-0 scoreline, decided the team that was named champion of the South Group and got promoted to the Alpha Ethniki.
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On the other hand, Pelopas Kiato, Olympiakos Neon Liosion, A.O. Patra and Panarkadikos got relegated to the Gamma Ethniki from the South Group. Pandramaikos, Edessaikos, P.O. Elassona and Odysseas Kordeliou were the teams that got relegated from the North Group.
South group final table
Pld = Matches played; W = Matches won; D = Matches drawn; L = Matches lost; GF = Goals for; GA = Goals against; GD = Goal difference; Pts = Points
Promotion play-off
The Promotion Play-off was played in Heraklion.
Rodos - Egaleo 1-0 (a.e.t.)