Van den Bergh reaction

Van den Bergh reaction is a chemical reaction used to measure bilirubin levels in blood. More specifically, it determines the amount of conjugated bilirubin in the blood. The reaction produces azobilirubin. Principle: bilirubin reacts with diazotised sulphanilic acid to produce purple coloured azo bilirubin.this reaction is highly useful in understanding the nature of jaundice. This was pioneered by the Dutch Physician, Abraham Albert Hijmans van den Bergh (1869–1943) of Utrecht.