Uihlein is a surname. Notable people with the surname include:
August Uihlein (1842–1911), German-born American businessmanJoseph E. Uihlein (1875–1968), American businessmanDavid Vogel Uihlein, Sr. (1920–2010), American heir and businessmanDavid Vogel Uihlein, Jr., American heir, businessman and conservative philanthropistLynde Bradley Uihlein (born 1945), American heiress and liberal philanthropistPeter Uihlein (born 1989), American golferRichard Uihlein (born 1945), American businessmanRobert Uihlein, Jr. (1916–1976), American businessmanWally Uihlein (born 1950), American golf businessman