Uihlein is a surname. Notable people with the surname include:
August Uihlein (1842–1911), German-born American businessman
Joseph E. Uihlein (1875–1968), American businessman
David Vogel Uihlein, Sr. (1920–2010), American heir and businessman
David Vogel Uihlein, Jr., American heir, businessman and conservative philanthropist
Lynde Bradley Uihlein (born 1945), American heiress and liberal philanthropist
Peter Uihlein (born 1989), American golfer
Richard Uihlein (born 1945), American businessman
Robert Uihlein, Jr. (1916–1976), American businessman
Wally Uihlein (born 1950), American golf businessman
