In enzymology, a tubulin-tyrosine ligase (EC 184.108.40.206) is an enzyme that catalyzes the chemical reaction
ATP + detyrosinated alpha-tubulin + L-tyrosine
alpha-tubulin + ADP + phosphate
The 3 substrates of this enzyme are ATP, detyrosinated alpha-tubulin, and L-tyrosine, whereas its 3 products are alpha-tubulin, ADP, and phosphate.
This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is alpha-tubulin:L-tyrosine ligase (ADP-forming).