Tryptophan N monooxygenase

Tryptophan N-monooxygenase (EC 1.14.13.125, tryptophan N-hydroxylase, CYP79B1, CYP79B2, CYP79B3) is an enzyme with systematic name L-tryptophan,NADPH:oxygen oxidoreductase (N-hydroxylating). This enzyme catalyses the following chemical reaction

L-tryptophan + 2 O₂ + 2 NADPH + 2 H⁺ ⇌ (E)-indol-3-ylacetaldoxime + 2 NADP⁺ + CO₂ + 3 H₂O (overall reaction) (1a) L-tryptophan + O₂ + NADPH + H⁺ ⇌ N-hydroxy-L-tryptophan + NADP⁺ + H₂O (1b) N-hydroxy-L-tryptophan + O₂ + NADPH + H⁺ ⇌ N,N-dihydroxy-L-tryptophan + NADP⁺ + H₂O (1c) N,N-dihydroxy-L-tryptophan ⇌ (E)-indol-3-ylacetaldoxime + CO₂ + H₂O

Tryptophan N-monooxygenase is a heme-thiolate protein (P-450).