In enzymology, a terephthalate 1,2-dioxygenase (EC 18.104.22.168) is an enzyme that catalyzes the chemical reaction
terephthalate + NADH + H+
(1R,6S)-dihydroxycyclohexa-2,4-diene-1,4-dicarboxylate + NAD+
The 4 substrates of this enzyme are terephthalate, NADH, H+, and O2, whereas its two products are (1R,6S)-dihydroxycyclohexa-2,4-diene-1,4-dicarboxylate and NAD+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor. The systematic name of this enzyme class is benzene-1,4-dicarboxylate,NADH:oxygen oxidoreductase (1,2-hydroxylating). Other names in common use include benzene-1,4-dicarboxylate 1,2-dioxygenase, and 1,4-dicarboxybenzoate 1,2-dioxygenase. This enzyme participates in 2,4-dichlorobenzoate degradation.