TRNA (pseudouridine54 N1) methyltransferase

TRNA (pseudouridine⁵⁴-N¹)-methyltransferase (EC 2.1.1.257, TrmY, m1Psi methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (pseudouridine54-N1)-methyltransferase. This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + pseudouridine⁵⁴ in tRNA ⇌ S-adenosyl-L-homocysteine + N¹-methylpseudouridine⁵⁴ in tRNA

This archaeal enzyme is specific for the 54 position.