TRNA (guanine9 N1) methyltransferase

TRNA (guanine⁹-N¹)-methyltransferase (EC 2.1.1.221, Trm10p, tRNA(m1G9/m1A9)-methyltransferase, tRNA(m1G9/m1A9)MTase, tRNA (guanine-N(1)-)-methyltransferase, tRNA m1G9-methyltransferase, tRNA m1G9 MTase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (guanine⁹-N¹)-methyltransferase. This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + guanine⁹ in tRNA ⇌ S-adenosyl-L-homocysteine + N¹-methylguanine⁹ in tRNA

The enzyme from Saccharomyces cerevisiae specifically methylates guanine⁹.