Speiser is a German surname. Notable people with the surname include:
Ambros Speiser (1922–2003), Swiss engineer and scientistAndreas Speiser (1885–1970), Swiss mathematicianEliyahu Speiser (1930–2009), Israeli politicianEphraim Avigdor Speiser (1902–1965), American AssyriologistFelix Speiser (1880-1949), Swiss ethnologistJerry Speiser (born 1953), Australian drummerMarkus Speiser (born 1985), Austrian footballerPaul Gustav Eduard Speiser (1877–1945), German entomologistPaul Speiser (1846–1935), Swiss politicianRosi Speiser (born 1951), German alpine skier