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Ste. Anne is a rural municipality lying southeast of Winnipeg, Manitoba, Canada. It is part of the Eastman Region and the Manitoba Census Division No. 2. Its population was 4,509 in the 2006 census, up from 4,427 in the 2001 census. The separately administered town of Ste. Anne lies within the borders of the municipality, in its northwestern part.
The Rural Municipality of Ste. Anne has five golf courses: The Links at Quarry Oaks Golf & Country Club, Cottonwood Golf and Country Club, Oakwood Golf Course, Ridgewood South Golf Course and the Girouxsalem Golf & Country Club.
The Rural Municipality of Ste. Anne includes Paradise Village, a 55 plus retirement community, which was founded in early 1990. Many of its residents are "snowbirds" who travel south in the winter.