Read (Biology)

In biology, genomics, transcriptomics, DNA-Seq or RNA-Seq context, "read" means a short sequence of dna, typically 25-400 base pairs long. Basically, reads are raw sequences that come off a sequencing machine. In the RNA-Seq methodology, RNA is converted in DNA (reverse transcription), fragmented and sequenced based on High-throughput sequencing technologies; the final result is millions of reads.