Samiksha Jaiswal (Editor)

Random hexamer

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A random hexamer or random hexonucleotides are for various PCR applications such as rolling circle amplification to prime the DNA.

They are oligonucleotide sequences of 6 bases which are synthesised entirely randomly to give a numerous range of sequences that have the potential to anneal at many random points on a DNA sequence and act as a primer to commence first strand cDNA synthesis.

References

Random hexamer Wikipedia