Ralph Warren was the son of Sir Thomas Warren of Feering, Essex, and grandson of William Warren.
Warren was a London mercer. He served as alderman, as Sheriff in 1528, and as Lord Mayor in 1536 and 1543. He was knighted in the first year of his mayoralty by Henry VIII.
He lived at Fulham House, a Grade II listed house at 87 Fulham High Street, Fulham.
Warren died on 11 July 1553, and was buried in the chancel of the church of St. Osythe's, (also known as St Benet Sherehog).
Warren married firstly Christiana Warcup, widow of Roger North (d.1509), and daughter of Richard Warcup or Warcop of Sinnington, Yorkshire. By her marriage to Roger North, Christiana (née Warcup) had a son and daughter, Sir Edward North, and Joan North, who married, and was the Marian exile, Joan Wilkinson.
Warren married secondly, Joan Trelake, the daughter of John Trelake alias Davy, of Cornwall, by whom she had two children, Richard Warren (d.1598) and Joan Warren (d.1572), who married her father's ward, Sir Henry Williams (later Cromwell) of Hinchinbrooke, Huntingdonshire, grandfather of the Protector Oliver Cromwell.
Joan married secondly, on 25 November 1558, Sir Thomas White, alderman of London, founder of St John's College, Oxford.