RRNA small subunit pseudouridine methyltransferase Nep1

RRNA small subunit pseudouridine methyltransferase Nep1 (EC 2.1.1.260, Nep1, nucleolar essential protein 1) is an enzyme with systematic name S-adenosyl-L-methionine:18S rRNA (pseudouridine¹¹⁹¹-N¹)-methyltransferase. This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + pseudouridine¹¹⁹¹ in yeast 18S rRNA ⇌ S-adenosyl-L-homocysteine + N¹-methylpseudouridine¹¹⁹¹ in yeast 18S rRNA

This enzyme recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal RNA based on the local RNA structure.