Pyruvate oxidase (CoA acetylating)

In enzymology, a pyruvate oxidase (CoA-acetylating) (EC 1.2.3.6) is an enzyme that catalyzes the chemical reaction

The 3 substrates of this enzyme are pyruvate, CoA, and O₂, whereas its 3 products are acetyl-CoA, CO₂, and H₂O₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with oxygen as acceptor. The systematic name of this enzyme class is pyruvate:oxygen 2-oxidoreductase (CoA-acetylating). This enzyme participates in pyruvate metabolism. It employs one cofactor, FAD.