PRIMOU is an enhancement of the PRIMO software program developed at the Genome Center at University of Texas Southwestern Medical Center at Dallas to choose primers and to calculate their annealing temperature according to the base stacking method. It has been modified by the University of Oklahoma to fix memory leaks and handle multi-megabase projects. It is currently maintained by Bruce Roe, and Steve Kenton at the University of Oklahoma.
References
Primou Wikipedia(Text) CC BY-SA