Neha Patil (Editor)

Polynucleotide 5' hydroxyl kinase

Updated on
Edit
Like
Comment
Share on FacebookTweet on TwitterShare on LinkedInShare on Reddit
EC number
  
2.7.1.78

IntEnz
  
IntEnz view

ExPASy
  
NiceZyme view

CAS number
  
37211-65-7

BRENDA
  
BRENDA entry

KEGG
  
KEGG entry


Medical vocabulary what does polynucleotide 5 hydroxyl kinase mean


In enzymology, a polynucleotide 5'-hydroxyl-kinase (EC 2.7.1.78) is an enzyme that catalyzes the chemical reaction

Contents

ATP + 5'-dephospho-DNA ⇌ ADP + 5'-phospho-DNA

Thus, the two substrates of this enzyme are ATP and 5'-dephospho-DNA, whereas its two products are ADP and 5'-phospho-DNA. Polynucleotide kinase is a T7 bacteriophage (or T4 bacteriophage) enzyme that catalyzes the transfer of a gamma-phosphate from ATP to the free hydroxyl end of the 5' DNA or RNA. The resulting product could be used to end-label DNA or RNA, or in a ligation reaction.

Nomenclature

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:5'-dephosphopolynucleotide 5'-phosphotransferase. Other names in common use include:

  • ATP:5'-dephosphopolynucleotide 5'-phosphatase
  • PNK
  • polynucleotide 5'-hydroxyl kinase (phosphorylating),
  • 5'-hydroxyl polynucleotide kinase,
  • 5'-hydroxyl polyribonucleotide kinase,
  • 5'-hydroxyl RNA kinase,
  • DNA 5'-hydroxyl kinase,
  • DNA kinase,
  • polynucleotide kinase, and
  • polynucleotide 5'-hydroxy-kinase.

    • References

    Polynucleotide 5'-hydroxyl-kinase Wikipedia
    (Text) CC BY-SA