Petrou (Greek: Πέτρου) is a Greek surname. People with this surname include:
Maria Petrou (1953–2012), Greek-born British computer scientist
Kostas Petrou (born 1959), British boxer of Greek Cypriot descent
Nick Petrou (born 1996), British cyclist
Nicolas Petrou (born 1967), Cypriot-born American fashion designer
Sokratis Petrou (born 1979), Greek footballer
David Michael Petrou, American film producer
George Petrou, Greek conductor and pianist