Supriya Ghosh (Editor)

Para Nitrophenylphosphate

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Formula
  
C6H6NO6P

Para-Nitrophenylphosphate 5png

Why para-nitrophenol is more acidic than ortho-nitrophenol in hindi: Acidity of nitrophenol


para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA and conventional spectrophotometric assays. These enzymes catalyse the hydrolysis of pNPP to yellow para-nitrophenol. This product absorbs strongly at 405 nm and can be measured with a visible light spectrophotometer and mainly used in the study of kinetics of enzyme-substrate catalysis reaction catalysed by alkaline phosphatase enzyme in accordance with Lineweaver-Burk formula.Activity assays of alkaline phosphatase with p-nitrophenyl phosphate are terminated by the addition of NaOH. This serves two functions; first, it stops the enzyme-catalysed reaction by changing pH which is unsuitable for the enzyme (the enzyme is denatured at high pH and so unable to function). Second, it deprotonates the p-nitrophenol to give the yellow coloured p-nitrophenylate. The progress of the reaction is monitored by absorption spectrum of p-nitrophenolate (obtained upon deprotonation of p-nitrophenol).

Para-Nitrophenylphosphate A Paranitrophenyl phosphate pNPP is a generic phosphatase

PNPP+H2O → PNP + inorganic phosphate

Para-Nitrophenylphosphate pNITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE CAS 4264839

Para-Nitrophenylphosphate paraNitrophenylphosphate Wikipedia

Para-Nitrophenylphosphate httpsuploadwikimediaorgwikipediacommonsthu

Para-Nitrophenylphosphate Use of MoO 2 Cl 2 DMF 2 as a precursor for molybdate promoted

Para-Nitrophenylphosphate pNitrophenyl Phosphate Disodium Salt Hexahydrate Fisher

Para-Nitrophenylphosphate pNitrophenyl phosphate dianion C6H4NO6P ChemSpider

Para-Nitrophenylphosphate Scheme 2 Hydrolysis of paranitrophenylphosphate pNPP to

References

Para-Nitrophenylphosphate Wikipedia


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