Ouseley or Ousley is a surname. Notable people with the surname include:
Frederick Gore Ouseley (1825–1889), English musician
Gideon Ouseley (1769–1839), Anglo-Irish Methodist
Gideon Ouseley, a pseudonym of Oliver St. John Gogarty (1878–1957)
Gore Ouseley (1770–1844), British diplomat and linguist
Herman Ouseley, Baron Ouseley (born 1945), British politician
author of the Ouseley Report
William Ouseley (1769–1842), British orientalist
William Gore Ouseley (1797–1866), British diplomat