Mylne is a surname, and may refer to:
Alfred Mylne (1871–1951), Scottish yacht designer
John Mylne (d.1621), Scottish master mason
John Mylne (d.1657), "John Mylne of Perth", Scottish master mason
John Mylne (1611–1667), "John Mylne junior", Scottish architect and master mason
Louis George Mylne (1843–1941), Anglican Bishop of Bombay from 1876 to 1897
Robert Mylne (1633–1710), Scottish architect and master mason
Robert Mylne (1733–1811), Scottish architect and engineer
Robert William Mylne (1817–1890), British architect and geologist
Walter Myln (d. 1558), Scottish Protestant martyr
William Mylne (1734–1790), Scottish architect and engineer
William Chadwell Mylne (1781–1863), British engineer and architect