Mycothiol dependent formaldehyde dehydrogenase

In enzymology, a mycothiol-dependent formaldehyde dehydrogenase (EC 1.2.1.66) is an enzyme that catalyzes the chemical reaction

The 3 substrates of this enzyme are formaldehyde, mycothiol, and NAD⁺, whereas its 3 products are S-formylmycothiol, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is formaldehyde:NAD+ oxidoreductase (mycothiol-formylating). This enzyme is also called NAD/factor-dependent formaldehyde dehydrogenase.