Mitsuo (written: 光男, 光央, 光雄, 光夫, 満男, 三男, 三雄, 美津雄, 己津央 or みつお in hiragana) is a masculine Japanese given name. Notable people with the name include:
Mitsuo Aoki (1914–2010), American theologian
Mitsuo Fuchida (淵田 美津雄) (1902–1976), Japanese naval aviator
Mitsuo Fukuda (福田 己津央) (born 1960), Japanese anime director
Mitsuo Harada (born 1964), Japanese golfer
Mitsuo Hashimoto (director) (橋本 みつお), Japanese anime director
Mitsuo Hashimoto (manga artist) (はしもと みつお) (born 1955), Japanese manga artist
Mitsuo Horigome (born 1974), Japanese cross-country skier
Mitsuo Horiuchi (堀内 光雄) (born 1930), Japanese politician
Mitsuo Ikeda (池田 三男) (1935–2002), Japanese sport wrestler
Mitsuo Iso (磯 光雄) (born 1966), Japanese animator and anime director
Mitsuo Itoh (伊藤 光夫) (born 1937), Japanese motorcycle racer
Mitsuo Iwata (岩田 光央) (born 1967), Japanese voice actor
Mitsuo Kagawa (賀川 光夫) (1923–2001), Japanese archaeologist and academic
Mitsuo Kamata (鎌田 光夫) (born 1937), Japanese footballer and manager
Mitsuo Kato (加藤 光雄) (born 1953), Japanese footballer
Mitsuo Matayoshi (又吉 光雄) (born 1944), Japanese activist
Mitsuo Mitani (三谷 光男) (born 1959), Japanese politician
Mitsuo Momota (百田 光雄) (born 1948), Japanese professional wrestler
Mitsuo Nakamura (中村 光夫) (1911–1988), pen-name of Koba Ichiro, Japanese writer
Mitsuo Nakanishi (中西 光雄) (born 1948), Japanese sprint canoeist
Mitsuo Ogasawara (小笠原 満男) (born 1979), Japanese footballer
Mitsuo Senda (千田 光男) (born 1940), Japanese voice actor
Mitsuo Shindō (信藤 三雄) (born 1948), Japanese art, film and music director
Taketani Mitsuo (武谷 三男) (1911–2000), Japanese physicist
Mitsuo Tsukahara (塚原 光男) (born 1947), Japanese artistic gymnast
Mitsuo Watanabe (渡辺 三男) (born 1953), Japanese footballer
Mitsuo Yanagimachi (柳町 光男) (born 1945), Japanese screenwriter and film director
Mitsuo Terada (寺田 光男) (born 1968), Tsunku (ja:つんく♂)'s real name, a prolific Japanese record producer, songwriter, and vocalist