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Middlebrook 7H10 Agar

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Middlebrook 7H10 Agar httpscataloghardydiagnosticscomcpprodConte

Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.

Composition

  • Ammonium Sulfate
  • Monopotassium phosphate
  • Disodium phosphate
  • Sodium Citrate
  • Magnesium Sulfate
  • sodium
  • Zinc Sulfate
  • Copper Sulfate
  • L-Glutamic Acid (sodium salt)
  • Ferric Ammonium Citrate
  • Pyridoxine Hydrochloride
  • Biotin
  • Malachite Green
  • Agar

  • Cultures should be read within 5-7 days after inoculation and once a week thereafter for up to 8 weeks.

    References

    Middlebrook 7H10 Agar Wikipedia