Middlebrook is a surname. Notable people with the surname include:
Diane Middlebrook (1939–2007), American biographer, poet and teacherJames Middlebrook (born 1977), English cricketerJason Middlebrook (born 1975), American baseball pitcherLindsay Middlebrook (born 1955), Canadian ice hockey goaltenderMartin Middlebrook (born 1932), British military historian and Fellow of the Royal Historical SocietyPeter Middlebrook (born 1969), English political economistR. D. Middlebrook (1929–2010), early pioneer of power electronicsWilliam Middlebrook, 1st Baronet (1851–1936), English solicitor and Liberal Party politicianWillie Middlebrook (1858–1919), English cricketerWillie Middlebrook (artist) (1957–2012), African-American photographer, artist and advocate