Middlebrook is a surname. Notable people with the surname include:
Diane Middlebrook (1939–2007), American biographer, poet and teacher
James Middlebrook (born 1977), English cricketer
Jason Middlebrook (born 1975), American baseball pitcher
Lindsay Middlebrook (born 1955), Canadian ice hockey goaltender
Martin Middlebrook (born 1932), British military historian and Fellow of the Royal Historical Society
Peter Middlebrook (born 1969), English political economist
R. D. Middlebrook (1929–2010), early pioneer of power electronics
William Middlebrook, 1st Baronet (1851–1936), English solicitor and Liberal Party politician
Willie Middlebrook (1858–1919), English cricketer
Willie Middlebrook (artist) (1957–2012), African-American photographer, artist and advocate