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An integrated microfluidic cell culture system for high throughput perfusion three dimensional cell
Microfluidics refers to a set of technologies for the manipulation for the small fluid volumes within artificially fabricated microsystems.And cell culture refers to the maintenance and growth of cells in a controlled laboratory environment. Cells are cooperatively regulated by numerous signals in their surrounding microenvironment. Microfluidic cell culture attempts to develop devices and techniques of culturing,maintaining,analyzing and experimenting in cells in micro scale volumes and facilitate simultaneous manipulation and analysis of cultured cells.The use of integrated microfluidic devices has advanced the fields of quantitative and systems in biology. Understanding the interplay between critical cell culture parameters and the micro environmental conditions created by microfluidic devices will accelerate the microfluid cell culture technology. The dimensions of the microfuidic channels are well suited to the physical scale of the biological cells.
Contents
- An integrated microfluidic cell culture system for high throughput perfusion three dimensional cell
- On chip co2 control for microfluidic cell culture
- Advantages
- References
On chip co2 control for microfluidic cell culture
Advantages
Microfluidics provide high degree control over cell culture conditions in various aspects. Given the small geometrical dimensions in microfluidics, the movements of fluids is laminar,and placement of fluid volumes in the nL-range, pL-range and even n fL-range is possible.The ability of exactly timing fluid flow using in-chip membrane valve allows precise chemical and physical control of the microenvironment.The small dimensions of spatially separated microfluid compartments allow assembly of a multitude of individually controllable cell culture chambers on a single device.Stem cells that are difficult to culture with conventional techniques can be expanded relatively rapidly in microfluidic culture.Non-dividing or slowly dividing cells can be culture continuously in the same cell culture chamber by regular or constant replacement of media. There are some advantages of microfluidic culture over macroscopic culture.That is in culture in flasks,dishes and well plates.There is great flexibility in the design of micro fluidic devices.
