Methylenediurea deaminase

Methylenediurea deaminase (EC 3.5.3.21, methylenediurease) is an enzyme with systematic name methylenediurea aminohydrolase. This enzyme catalyses the following chemical reaction

methylenediurea + 2 H₂O ⇌ N-(hydroxymethyl)urea + 2 NH₃ + CO₂ (overall reaction) (1a) methylenediurea + H₂O ⇌ N-(carboxyaminomethyl)urea + NH₃ (1b) N-(carboxyaminomethyl)urea ⇌ N-(aminomethyl)urea + CO₂ (spontaneous) (1c) N-(aminomethyl)urea + H₂O ⇌ N-(hydroxymethyl)urea + NH₃ (spontaneous)

Methylenediurea is hydrolysed and decarboxylated to give an aminated methylurea.