Maysville and Lexington Railroad, Southern Division

The Maysville and Lexington Railroad, Southern Division, was a 19th- and early 20th-century railway company in north-central Kentucky in the United States. In 1868, along with the Northern Division, it restored the service of the earlier Maysville & Lexington line, which had failed in 1856. The Southern Division was more successful than the Northern, which failed in 1875 and was reörganized as the "North Division". The Southern line survived until 1921, when it and the North Division were purchased by the L&N.

The Southern Division's routes and rights-of-way are today owned by CSX Transportation.