Mancuso is a surname. An alternate spelling can be Mancusi. Notable people with the surname include:
Alejandro Mancuso, retired Argentine footballer
Anna Mancuso, Canadian former politician
Ciro Mancuso, Nevada-based drug dealer convicted of running a $140-million marijuana smuggling operation
David Mancuso, American DJ
Filippo Mancuso, Italian judge and politician
Frank Mancuso Jr., movie producer
Gus Mancuso, catcher in Major League Baseball from 1928 to 1945
Julia Mancuso, American alpine skier who won a gold medal at the 2006 Winter Olympics
Laurence Mancuso, founding abbot of the New Skete monastery
Leonardo Mancuso, Italian footballer
Nick Mancuso, Italian-Canadian cinema and stage actor
Renee Mancuso, American bridge player
Salvatore Mancuso, Colombian paramilitary warlord
The fictional character Nick Mancuso, played by Robert Loggia in Favorite Son (1988) and Mancuso, F.B.I. (1989)