Luigi Agnolin (born 21 March 1943 in Bassano del Grappa) is a former football referee from Italy. He is mostly known for supervising four matches in the FIFA World Cup, one in 1986 and three in 1990. He also refereed the 1988 European Cup Final between PSV Eindhoven and S.L. Benfica.
In addition to his two World Cup tournaments, Agnolin also served as a referee in qualifiers for the World Cups of 1982, 1986, and 1990. He officiated in qualifying for Euro 1984. Agnolin is known to have worked as a FIFA referee during the period from 1980 to 1990.
Agnolin was also a futsal referee, officiating at the 1989 FIFA Futsal World Championship in the Netherlands.
In 2012, he was inducted into the Italian Football Hall of Fame.