Lipoyl synthase

In enzymology, a lipoyl synthase (EC 2.8.1.8) is an enzyme that catalyzes the chemical reaction

protein N₆-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine ⇌ protein N₆-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine

The 3 substrates of this enzyme are protein N6-(octanoyl)lysine, sulfur, and S-adenosyl-L-methionine, whereas its 3 products are protein N6-(lipoyl)lysine, L-methionine, and 5'-deoxyadenosine.

This enzyme belongs to the family of transferases, specifically the sulfurtransferases, which transfer sulfur-containing groups. This enzyme participates in lipoic acid metabolism, where it performs the final step in lipoic acid biosynthesis.