Levene is a surname. Notable people with the surname include:
Allan Levene, (born 1949 or 1950), British American information technology specialist and political candidate
George Levene (1885–1930), American football player and coach at the college level
Gus Levene (1911-1979), American composer, arranger, orchestrator, guitarist
Gustavo Gabriel Levene (1905–1987), Argentinian historian and writer
Harry Levene, English boxing promoter, associated with Terry Lawless
John Levene (born 1941), English film and television actor
Keith Levene (born 1957), English guitarist and songwriter
Peter Levene (born 1941), English businessman and nobleman
Phoebus Levene (1869–1940), biochemist who identified the components of DNA
Rebecca Levene, British author
Sam Levene (1905–1980), American stage and film actor