Layston is a former village and parish located about a kilometre north-east of Buntingford in Hertfordshire, at 51°57′50″N 0°00′45″E.
Its former church, St Bartholomew's, became derelict but is the subject of a restoration project. People connected with the church include:
Thomas Crouch, who was baptised in St Bartholomew's in 1607
Robert Wogdon (January 1734 – 28 March 1813), founder of the gunsmith firm Wogdon & Barton, who was buried in the chancel with his wife, who had died in 1805
Rev. Jonathan Gilder and Mary Brazier, who were married there in 1759; their daughter Sarah, baptised there in 1767, became (posthumously) the maternal grandmother to New Zealand suffragette Catherine Fulton
Claud Lovat Fraser (15 May 1890 – 18 June 1921), who was buried in the churchyard.
There is a state-funded primary school, Layston First CE School.
