Knowlton is a surname. Notable people with the surname include:
Andrew Knowlton, magazine editor and television personality
Austin Eldon Knowlton (1909–2003), architect that spent most of his career in the construction industry
Charles Knowlton (1800–1850), American physician and writer
Daniel Gibson Knowlton, American bookbinder at Brown University 1956-1992
David Clark Knowlton, American social anthropologist and contributor to Mormonism: A Historical Encyclopedia
Ebenezer Knowlton, co-founder of Bates College, Congressman, abolitionist, and Free Will Baptist minister
Ken Knowlton a computer graphics pioneer, artist, mosaicist and portraitist, who worked at Bell Labs
Miles Justice Knowlton, American Baptist missionary to China
Richard Knowlton, former CEO of the Hormel company
Thomas Knowlton (1740–1776), American patriot who served in the French and Indian War and acted as a Colonel during the American Revolution
William A. Knowlton (1920–2008), General
Wiram Knowlton (1816–1863), American politician
