Karachi University cricket team, representing the University of Karachi in Karachi, played first-class cricket in Pakistan from 1958-59 to 1967-68, never losing a match.
Karachi University first played first-class cricket when the four-team Inter-Universities Championship was given first-class status in 1958-59 and 1959-60. They won in both years, beating Punjab University in the final each time.
Karachi University, along with Punjab University, then competed at first-class level in the Ayub Trophy in 1960-61, 1964-65, 1965-66 and 1967-68. They reached the semi-finals in 1960-61, when in their three matches Afaq Hussain took 29 wickets at 12.82.
Overall between 1959 and 1968 Karachi University played 13 first-class matches, winning 8 and drawing 5.